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RNA-Seq Shows its Sensitive Side
(runtime: 2:17 min)
 
 
   
     
  Next generation sequencing has led to diverse RNA-Seq methods, but one drawback is sensitivity. Conventional RNA-Seq requires >10 ng of RNA from 50–100 cells. But increasingly researchers are interested in profiling gene expression in single cells. Ramskold and colleagues used the SMARTer® Ultra Low Input RNA Kit for Illumina® sequencing to investigate gene expression in a single circulating tumor cell, starting with a mere 10 pg of RNA. — That's Good Science!  
     
     

頁面更新:2021-01-20 08:48:11