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當前位置：首頁> 產(chǎn)品選擇指南 > 干細胞研究相關制品 > 細胞培養(yǎng)產(chǎn)品 (Cell culture) > 人源干細胞增殖用培養(yǎng)基Human Stem Cell Culture System (DEF-CS, all-in-one format)
人源干細胞增殖用培養(yǎng)基Human Stem Cell Culture System (DEF-CS, all-in-one format)
品牌 Code No. 產(chǎn)品名稱 包裝量 價格（元） 說明書 數(shù)量
Cellartis Y30010 Cellartis® DEF-CS™ 500 Culture System 1 Kit ￥6,546
無標題文檔
Human iPS和Human ES增殖用培養(yǎng)基
(DEF-CS Culture System, all-in-one format)
作為一款高端創(chuàng)新型的產(chǎn)品，Cellartis DEF-CS培養(yǎng)系統(tǒng)使得單細胞干細胞操作成為常規(guī)的操作。該產(chǎn)品是專門針對人類誘導干細胞（hiPS）和人類胚胎干細胞（hES）研發(fā)的高效率增殖用培養(yǎng)基產(chǎn)品體系。該產(chǎn)品系統(tǒng)為all-in-one型，包括了所有組分，客戶不需要另外求購組分。該產(chǎn)品是成分確定的培養(yǎng)基產(chǎn)品，而且不需要飼養(yǎng)層。DEF-CS既可以實現(xiàn)單細胞培養(yǎng)，也可以用于傳統(tǒng)的iPS培養(yǎng)模式以及大規(guī)模干細胞增殖。在使用DEF-CS培養(yǎng)基增殖干細胞時，幾乎沒有背景分化的問題，這使得細胞篩選不再必需。作為一款創(chuàng)新產(chǎn)品，利用DEF-CS培養(yǎng)干細胞時，可以使用酶消化法（enzymatic passaging）實現(xiàn)需要單細胞操作，這一特點十分有利于高通量細胞鑒別篩選（high-throughput screening）、轉染（transfection）、框架接種（scaffold seeding）等。
 
■ 產(chǎn)品特點
· Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation
 
■ 產(chǎn)品應用
· Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold)
 
■ 產(chǎn)品詳情請點擊：
 
Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems.
 
參考文獻：
1. Sivertsson, Louise, et al. "Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor." Stem cells and development 22.4 (2012): 581-594.
2. Hanson, Charles, et al. "Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro." Acta ophthalmologica 91.2 (2013): 127-130.
3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level." Methods 59.1 (2013): 59-70.
4. Ulvestad, Maria, et al. "Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells." Biochemical pharmacology 86.5 (2013): 691-702.
5. Ramirez JM, et al. Side scatter intensity is highly heterogeneous in undifferentiated pluripotent stem cells and predicts clonogenic self-renewal. Stem Cells Dev.2013 Jun 15;22(12):1851-1860.
6. Borestrom, Cecilia, et al. “Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source.” Stem Cells Trans. Med. (2014) 3, 433-447.
7. Kia, Richard, et al. "MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity." Toxicological Sciences (2014): kfu269.
8. Valton, Julien, et al. "Efficient strategies for TALEN-mediated genome editing in mammalian cell lines."  Methods 69.2 (2014): 151-170.
9. Zandén, Carl, et al. "Stem cell responses to plasma surface modified electrospun polyurethane scaffolds." Nanomedicine: Nanotechnology, Biology and Medicine 10.5 (2014): 949-958.
10. Asplund, Annika, et al. “One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying? Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells” Stem Cell Rev and Rep (2015)
 
 
 
 

頁面更新：2024-01-25 16:05:42