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當前位置：首頁> 產(chǎn)品選擇指南 > 干細胞研究相關(guān)制品 > 細胞培養(yǎng)產(chǎn)品 (Cell culture) > 神經(jīng)干細胞培養(yǎng)基Adherent Neural Stem Cell Culture Media
神經(jīng)干細胞培養(yǎng)基Adherent Neural Stem Cell Culture Media
品牌 Code No. 產(chǎn)品名稱 包裝量 價格（元） 說明書 數(shù)量
Cellartis Y40001 RHB-A® 500 ml ￥4,338
無標題文檔
Neural Stem Cell Culture Media
RHB-A和RHB-Basal是兩款無血清、成分完全確定的培養(yǎng)基，用于人類或小鼠神經(jīng)干細胞（NS）的定向分化獲取、維持培養(yǎng)、擴增、誘導分化成神經(jīng)細胞等用途。RHB-A可用來培養(yǎng)擴增貼壁的人或小鼠的神經(jīng)干細胞。在RHB-A中培養(yǎng)的神經(jīng)干細胞保持著穩(wěn)定的神經(jīng)干細胞分化發(fā)育成為成熟的神經(jīng)細胞的能力 (1-3) 。另外，RHB-A還用來定向分化小鼠胚胎干細胞(mouse ES)至神經(jīng)前體細胞 (neural precursors) (4-6)。
RHB‐Basal不包含生長因子或是神經(jīng)細胞相關(guān)的添加物。因此，可以RHB‐Basal為基礎(chǔ)，通過添加客戶認為需要的添加物來開發(fā)適合客戶的特殊類型細胞的培養(yǎng)基。
 
■ 產(chǎn)品特點
· RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells
· RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements
 
■ 產(chǎn)品應用
· Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
· Maintenance and propagation of adherent mouse and human NS cells
· Differentiation of mouse and human NS cells into functional neurons
· Differentiation of mouse ES cells to neuronal precursors
· Refer to the Data Sheet for additional examples of use
 
■ 產(chǎn)品詳情請點擊：
 
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).
 
參考文獻：
1. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nature Biotechnology 21:183-186.
2. Conti L, et al. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology 3(9):e283.
3. Pollard SM, et al. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex 16:112-120. 4. Diogo MM, et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnology and Applied Biochemistry 49:105-112.
5. Pollard SM, et al. (2008) Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience 38:393:403.
6. Sun Y, et al. (2008) Long-term tripotent differentiation capacity of human neural stem (NS)cells in adherent culture. Molecular and Cellular Neuroscience 38:245-258.
7. Pollard SM, et al. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell 4:568-580.
8. Abranches E, et al. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE 4(7): e6286.
9. Fernandes et al. (2010) Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells. Biotechnology and Bioengineering 106: 260–270.
10. Fernandes, T.G., et al. (2010) Different stages of pluripotency determine distinct patterns of proliferation, metabolism, and lineage commitment of embryonic stem cells under hypoxia. Stem Cell Research 5(1):76-89.
11. Fernandes et al. (2010) Three-dimensional cell culture microarray for high-throughput studies of stem cell fate. Biotechnology and Bioengineering 106: 106-118.
12. Hansen, et al. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature doi: 10.1038/nature08845.
13. Hook L, et al. (2011) Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays. Neurochem Int. 59(3): 432-44.
 
 

頁面更新：2024-02-29 10:56:09