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當(dāng)前位置：首頁> 產(chǎn)品選擇指南 > 干細(xì)胞研究相關(guān)制品 > 細(xì)胞培養(yǎng)產(chǎn)品 (Cell culture) > 臨床前擴(kuò)增人干細(xì)胞培養(yǎng)基Human Stem Cell Culture Medium (DEF-CS Xeno-Free)
臨床前擴(kuò)增人干細(xì)胞培養(yǎng)基Human Stem Cell Culture Medium (DEF-CS Xeno-Free)
品牌 Code No. 產(chǎn)品名稱 包裝量 價(jià)格（元） 說明書 數(shù)量
Cellartis Y30045 Cellartis® DEF-CS™ 500 Xeno-Free Culture Medium w/o antibiotics 1 Kit ￥3,719
Cellartis Y30071 Cellartis® DEF-CS™ 500 Xeno-Free GMP Grade Basal Medium (Prototype) 500 ml 詢價(jià)
無標(biāo)題文檔
臨床前擴(kuò)增人干細(xì)胞培養(yǎng)基系列（Xeno-free)
(The Xeno-Free Culture Media Product Family for Preclinical Expansion of Human iPS Cells)
Cellartis DEF-CS (Xeno-free) 培養(yǎng)基系列，完全無任何人源及動(dòng)物源成分，被用于人類誘導(dǎo)多能干細(xì)胞 (iPSCs) 擴(kuò)增。該款產(chǎn)品不使用飼養(yǎng)層 (feeder)，而是使用xeno-free的基質(zhì)物，比如 新型的iMatrix-511。使用DEF-CS 培養(yǎng)基 (xeno-free) 培養(yǎng)的細(xì)胞，擁有高的增殖率和穩(wěn)定的核型，允許2D-單層培養(yǎng)和3D-懸浮培養(yǎng)進(jìn)行有效擴(kuò)增。
 
這些產(chǎn)品主要適用于：
· 基礎(chǔ)研究和臨床前概念研究—Cellartis DEF-CS 500 Xeno-Free Culture Medium幫助您從基礎(chǔ)研究過渡到下一階段相關(guān)疾病模型的驗(yàn)證和生產(chǎn)制造戰(zhàn)略的開展。
 
· 擴(kuò)增到足夠的細(xì)胞數(shù)—Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium搭配灌注生物反應(yīng)器系統(tǒng)，提供了一個(gè)產(chǎn)生大量高質(zhì)量的、未分化的、用作起始細(xì)胞治療材料的理想方法。
 
· GMP 級(jí)生產(chǎn)和下游使用于臨床研究—Cellartis DEF-CS 500 Xeno-Free GMP Grade Basal Medium (Prototype)遵循嚴(yán)格的生產(chǎn)準(zhǔn)則，確保沒有可能損害患者的人類和動(dòng)物來源的物質(zhì)。
 
■ 產(chǎn)品特點(diǎn)
Cellartis DEF-CS 500 Xeno-Free Culture Medium
· Maintains pluripotency and stable karyotype in a 2D non-colony monolayer culture
· Ideal for maintenance of undifferentiated pluripotent cells and scale-up to therapeutically relevant cell numbers
· Fully compatible with the GMP-grade version for a seamless transition to the clinic
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium
· Pluripotent cells in the spheroids retain full differentiation potential and stable karyotype
· Produces 5 × 109 cells in a 1 L bioreactor within 3–4 passages
· Provides a distinct advantage for scale-up to therapeutically relevant cell numbers
Cellartis DEF-CS 500 Xeno-Free GMP Grade Basal Medium (Prototype)
· Optimized for small- or large-scale production of pluripotent cells for downstream use in clinical research settings
· Manufactured as a quality-assured product, according to the guidelines for GMP for investigational products
· Rigorous manufacturing standards ensure quality and consistency; all materials are of clinical quality, with traceable production processes
 
■ 產(chǎn)品應(yīng)用
· Culturing human iPS cells in completely xeno-free conditions for preclinical studies.
 
■ 產(chǎn)品目錄
Code No. Product Size
Y30045 Cellartis® DEF-CS™ 500 Xeno-Free Culture Medium w/o antibiotics 1 kit
Y30042 Cellartis® DEF-CS™ 500 Xeno-Free Additives 1 kit
Y30046 Cellartis® DEF-CS™ 500 Xeno-Free Basal Medium w/o antibiotics 500 ml
 
Code No. Product Size
Y30046 Cellartis® DEF-CS™ 500 Xeno-Free Basal Medium w/o antibiotics 500 ml
 
Code No. Product Size
Y30071 Cellartis® DEF-CS™ 500 Xeno-Free GMP Grade Basal Medium (Prototype) 500 ml
 
■ 關(guān)聯(lián)產(chǎn)品
Code No. Product Size
T303 iMatrix-511 2 × 175 μg
 
■ 產(chǎn)品詳情請(qǐng)點(diǎn)擊
Y30045：
Y30071：
 
High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates grown under 4% O2 perfusion conditions were sequentially passaged three times by mechanical disruption (protocol adapted from Otsuji et al. 2014); cells were counted at passage on days 4 and 7, and at the final time point on day 11, to determine the expansion factor relative to the initial seeding number.
 
High-quality hiPS cell aggregates were grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). After expansion of hiPS cells as aggregates, cells were harvested on day 4 and expression of OCT4, SSEA-4, TRA-1-60 (pluripotency markers) and SSEA-1 and SOX17 (differentiation markers) was analyzed by immunostaining.
 
  Takara Bio USA, Inc.比較結(jié)果

頁面更新：2024-12-02 13:37:15

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