The Cellartis Definitive Endoderm Differentiation Kit用于定向分化hiPS獲取definitive endoderm (DE) cells。本產(chǎn)品包含所有必需的培養(yǎng)基和coating材料。本產(chǎn)品還包括新一代的Cellartis DEF-CS 100 Culture System用于起始hiPS的維持培養(yǎng)用。使用本產(chǎn)品獲得的DE細(xì)胞,可以作為起始材料進(jìn)行定向分化至內(nèi)胚層來源的細(xì)胞類型(如肝細(xì)胞,胰腺細(xì)胞)。Cellartis也提供分化DE細(xì)胞獲取肝細(xì)胞的試劑盒(Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050);Cellartis也提供誘導(dǎo)分化hiPS獲取肝細(xì)胞的試劑盒(Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)。
■ 產(chǎn)品特點(diǎn)
· Efficient differentiation—generates >80% SOX17-positive cells
· Robust protocol—successful definitive endoderm (DE) cell generation from more than 25 different hiPS cell lines
· Complete kit—media and ready-to-use coating for a seven-day protocol to reproducibly differentiate hiPS cells into DE cells
· Start with a homogeneous population of hiPS cells—kit includes the Cellartis DEF-CS 100 Culture System to maintain undifferentiated cells prior to the start of DE cell differentiation
■ 產(chǎn)品詳情請點(diǎn)擊:
mRNA expression profiles of definitive endoderm cells being generated from two hiPS cell lines (ChiPSC18 and P11025) using the Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture Medium. These graphs track the relative temporal mRNA expression levels of various markers during differentiation from hiPS cells (starting on Day 0) into DE cells (by Day 7), as measured by RT-qPCR.