Probe qPCR Mix (2X)*1 |
1.0 ml × 5 |
ROX Reference Dye (50X)*2 |
200 μl × 1 |
ROX Reference Dye II (50X)*2 |
200 μl × 1 |
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Probe qPCR Mix, with UNG (Code No.: RR392A,50 μl反應(yīng)×200次) |
Probe qPCR Mix, with UNG (2X)*3 |
1.0 ml × 5 |
ROX Reference Dye (50X)*2 |
200 μl × 1 |
ROX Reference Dye II (50X)*2 |
200 μl × 1 |
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*1 內(nèi)含PCR酶,dNTP Mixture,Mg2+,Tli RNaseH。
*2 使用在Applied Biosystems的Real Time PCR擴(kuò)增儀上,用以校正孔與孔之間產(chǎn)生的熒光信號(hào)誤差。
◆ 使用ROX Reference Dye (50×) 的PCR儀 (使用時(shí)終濃度為1×)
7300 Real-Time PCR System
StepOnePlus Real-Time PCR System (以上Thermo Fisher Scientific)
◆ 使用ROX Reference Dye II (50×) 的PCR儀 (使用時(shí)終濃度為0.5×)
7500 Real-Time PCR System
7500 Fast Real-Time PCR System (以上Thermo Fisher Scientific)
◆ 不需要使用ROX的PCR儀
Thermal Cycler Dice Real Time System III(Code No. TP950/TP970/TP980/TP990)
Thermal Cycler Dice Real Time System II /Lite(Code No.TP900/TP960、TP700/TP760:終賣)
LightCycler 96/480 System(Roche Diagnostics)
CFX96 Real-Time PCR Detection System (Bio-Rad)
Smart Cycler System/Smart Cycler II System(Cepheid)
*3 內(nèi)含PCR酶,dNTP Mixture,Mg2+,Tli RNase H和UNG。
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