Fast Probe qPCR mix (TCH005:50 μl反應×200次量;TCH009:50 μl反應×40次量) |
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TCH005 |
TCH009 |
Fast Probe qPCR Mix (2X)*1 |
1 ml×5 |
1 ml |
ROX Reference Dye (50X Conc.)*3 |
200 μl |
40 μl |
ROX Reference Dye II (50X Conc.)*3 |
200 μl |
40 μl |
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Fast Probe qPCR mix , with UNG(TCH006:50 μl反應×200次量;TCH010:50 μl反應×40次量) |
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TCH006 |
TCH010 |
Fast Probe qPCR mix (2X), with UNG*2 |
1 ml×5 |
1 ml |
ROX Reference Dye (50X Conc.)*3 |
200 μl |
40 μl |
ROX Reference Dye II (50X Conc.)*3 |
200 μl |
40 μl |
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*1 內(nèi)含PCR酶,dNTP Mixture,Mg2+,Tli RNase H。
*2 內(nèi)含PCR酶,dNTP Mixture,Mg2+,Tli RNase H和UNG。
*3 使用在Applied Biosystems等Real Time PCR擴增儀上,用以校正孔與孔之間產(chǎn)生的熒光信號誤差。
◆ 使用ROX Reference Dye(50X)的PCR儀
StepOnePlus Real-Time PCR System(Thermo Fisher Scientific)
◆ 使用ROX Reference Dye II(50X)的PCR儀
7500 Fast Real-Time PCR System
Applied Biosystems™ QuantStudio 3/5 Real-Time PCR System(以上Thermo Fisher Scientific)
◆ 不需要使用ROX的PCR儀
Thermal Cycler Dice™ Real Time System IV (Code No. TP1000)
Thermal Cycler Dice Real Time System III(Code No. TP950/TP970/TP980/TP990)
Thermal Cycler Dice Real Time System II/Lite(Code No. TP900/TP960、TP700/TP760:終賣)
CronoSTAR™ 96 Real-Time PCR System (Code No. 640231/640232)
LightCycler 96/480 System(Roche Diagnostics)
CFX96 Real-Time PCR Detection System(Bio-Rad)
Gentier 96E/96R 全自動醫(yī)用PCR分析系統(tǒng) (西安天隆科技)
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