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當(dāng)前位置：首頁> 產(chǎn)品選擇指南 > cDNA合成 & 克隆 > 克隆學(xué)習(xí)中心 > 高效的In-Fusion無縫克隆試劑盒
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>> 升級的無縫克隆試劑In-Fusion Snap Assembly
 
 
 
 
 
 
>> 看動畫，讓你更懂In-Fusion無縫克隆
2分鐘了解In-Fusion的操作流程
 
多才多藝的In-Fusion無縫克隆
 
2分鐘看懂In-Fusion作用機制的與眾不同
 
>> 自動化In-Fusion 克隆
  In-Fusion Snap Assembly可以通過簡單的步驟，實現(xiàn)無需限制酶、定向、準(zhǔn)確（95%）且背景非常低的克隆。In-Fusion克隆系統(tǒng)的簡單性和準(zhǔn)確性使其成為高通量克隆的理想選擇，同時不影響克隆的穩(wěn)定性。以下資料展示了在一個通用的液體處理平臺上的自動化In-Fusion克隆工作流程。
 
制造商 平臺 試劑盒 應(yīng)用 TBUSA Application Note
Beckman Beckman Coulter Biomek i7 自動化工作站 In-Fusion Snap Assembly Master Mix 使用96孔板進(jìn)行自動化In-Fusion 克隆   
Eppendorf Eppendorf epMotion® 5075t工作站 In-Fusion Snap Assembly Master Mix 使用96或384孔板進(jìn)行自動化In-Fusion 克隆   
Opentrons Opentrons OT-2 In-Fusion Snap Assembly Master Mix 使用96孔板進(jìn)行自動化In-Fusion 克隆   
 
>> In-Fusion客戶應(yīng)用實例
    ■ 利用In-Fusion Cloning插入小的融合蛋白質(zhì)標(biāo)簽
 
    ■ In-Fusion Cloning：連接酶的高效率高準(zhǔn)確度替代方法
 
    ■ In-Fusion Cloning：高效率的單片段和多片段克隆
 
    ■ In-Fusion Cloning：成功地直接克隆到大表達(dá)載體
 
    ■ In-Fusion Cloning：克服慢病毒載體酶切位點的限制
 
    ■ In-Fusion Cloning：更快速地得到比TOPO克隆更準(zhǔn)確的結(jié)果
 
    ■ In-Fusion Cloning：簡單的多片段克隆解決一個合成的難題
 
    ■ 利用In-Fusion Cloning System克隆2個PCR片段至載體
 
    ■ 利用In-Fusion Cloning System進(jìn)行3個插入片段與載體的多片段克隆
 
    ■ 利用In-Fusion 方法進(jìn)行4個插入片段與載體的多片段克隆
 
    ■ In-Fusion Cloning Kit與其他公司同類型試劑盒的比較
 
    ■ 使用In-Fusion Cloning System構(gòu)建基因編輯敲除用供體載體（3個插入片段的In-Fusion克隆）
 
>> In-Fusion應(yīng)用文獻(xiàn)例
    ■ CRISPR/Cas9載體克隆
    · Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system
    · A highly efficient ligation-independent cloning system for CRISPR/Cas9 based
       genome editing in plants
    · MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the
       PITCh systems
 
    ■ 抗體工程研究中的高通量克隆
    · Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells
 
    ■ 慢病毒載體克隆
    · Gene expression profiling reveals U1 snRNA regulates cancer gene expression
    · Single cell resolution in vivo imaging of DNA damage following PARP inhibition
    · Lrig1 is a haploinsufficient tumor suppressor gene in malignant glioma. Self-targeting
       of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors
    · Self-targeting of TNF-releasing cancer cells in preclinical models of primary and
       metastatic tumors
 
    ■ 合成DNA組裝
    · Initiation of translation in bacteria by a structured eukaryotic IRES RNA
    · Targeted mutagenesis of guinea pig Cytomegalovirus using CRISPR/Cas9-mediated
       gene editing
 
    ■ 高通量克隆
    · A versatile ligation-independent cloning method suitable for high-throughput
       expression screening applications
    · Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells
    · High throughput generation and characterization of replication-competent clade C
       transmitter-founder simian human immunodeficiency viruses
 
    ■ 多片段克隆
    · In-Fusion assembly: seamless engineering of multidomain fusion proteins, modular
       vectors, and mutations
    · Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a
       variety of lipid-linked glycans
    · Multi-homologous recombination-based gene manipulation in the rice pathogen
       Fusarium fujikuroi
    · Structural basis of pathogen recognition by an integrated HMA domain in a plant
       NLR immune receptor
    · Structure-function studies of an engineered scaffold protein derived from Stefin
       A.II: Development and applications of the SQT variant
    · Regulating prospero mRNA stability determines when neural stem cells stop dividing
    · Identification of the N-terminal domain of the influenza virus PA responsible for
       the suppression of host protein synthesis
    · Root developmental programs shape the Medicago truncatula nodule meristem
    · Towards a systems approach in the genetic analysis of archaea: accelerating
       mutant construction and phenotypic analysis in Haloferax volcanii
    · Potential pitfalls and solutions for use of fluorescent fusion proteins to study
       the lysosome
 
>> In-Fusion克隆工具箱
In-Fusion引物設(shè)計工具 In-Fusion體系摩爾比計算器
 
>> 實驗設(shè)計原則和技巧
    ■ 利用In-Fusion HD Cloning進(jìn)行堿基刪除、插入或替換
    ■ 利用In-Fusion HD Cloning進(jìn)行多片段克隆的實驗技巧
    ■ In-Fusion HD Cloning技巧、訣竅、常見問題和排查
    · FAQs
    · Tips
 
 
 

頁面更新：2024-05-15 13:41:21